Your alternative to RPA technology
Recombinase Aided Amplification for end-point detection of RNA and DNA
(RT-)RAA Nucleic Acid Amplification Kit (End-point Method) can be used to amplify nucleic acids. The amplification result can be verified by use of gel electrophoresis.
RAA technology is one of the fastest nucleic acid amplification methods and amplifies RNA and DNA in just a few minutes. For detection on an agarose gel, the amplification reaction can be stopped after 15-30min incubation.
Aliquoted freeze-dried reagent mixes
Recombinase Aided Amplification kits are convient to handle. They comprise of freeze-dried enzyme mix aliquoted in standard 0.2mL PCR tubes and of two buffers for rehydration and starting the amplification reaction.
Reverse transcriptase included
In the RT-version of RAA kits, the reverse transcriptase enzyme is included in the freeze-dried reagent pellet. There is no extra pipetting step and no extra costs.
Protocol for RAA assays and end-point detection:
- prepare mastermix of amplification buffer, magnesium acetate and primer mix
- open tube with freeze-dried reagents and add mastermix into the inner surface of the tube lid
- add extracted nucleic acids to the lid as well
- close tube, short-spin and mix by pulse-vortexing
- place tube in your heating block or instrument
- incubate reactions for 15-30 minutes (at approx. 37-42°C)
- optional: heat amplification reactions at 80°C for 5 minutes
- add the amplified nucleic acids to your agarose gel (2% recommended)