Your alternative to RPA technology
Recombinase Aided Amplification for real-time fluorescence detection
(RT-)RAA Nucleic Acid Amplification Kit (Fluorescent Method) can be used to amplify nucleic acids. The amplification result can be verified by use of fluorescent probes in real-time.
Rapid amplification and real-time detection
RAA technology amplifies RNA and DNA in just a few minutes. Positive amplification results are usually detectable after 3 to 10 minutes incubation, provided an appropriate fluorescence reader is used for incubation.
Aliquoted freeze-dried reagent mixes
Recombinase Aided Amplification kits are convient to handle. They comprise of freeze-dried enzyme mix aliquoted in standard 0.2mL PCR tubes and of two buffers for rehydration and starting the amplification reaction.
Reverse transcriptase included
In the RT-version of RAA kits, the reverse transcriptase enzyme is included in the freeze-dried reagent pellet. There is no extra pipetting step and no extra costs.
Protocol for fluorescent RAA assays:
- prepare mastermix of reagents buffer, magnesium acetate and primer-probe mix
- open tube with freeze-dried reagents and add mastermix into the inner surface of the tube lid
- add extracted nucleic acids to the lid as well
- close tube, short-spin and mix by pulse-vortexing
- place tube in your instrument and start incubation and detection (at approx. 37-42°C)
Virus-specific real-time detection assays available for:
|SARS-CoV-2||Japanese Encephalitis Virus|
|African Swine Fever (ASF) Virus||Ebola Virus|
|Yellow Fever Virus||Influenza A Virus|
|Zika Virus||Dengue Virus|
Further real-time detection assays available for:
|Mycobacterium tuberculosis||Vibrio cholerae|
|Plasmodium spp.||Vibrio parahemolyticus|
|Salmonella spp.||Bacillus anthracis|
|Listeria monocytogenes||Yersinia pestis|
|Staphylococcus aureus||Brucella spp.|
RAA kit for DNA amplification and real-time fluorescence detection
RT-RAA kit for RNA amplification and real-time fluorescence detection
Real-time assays for numerous pathogens, e.g. SARS-CoV-2, MTB, Dengue, Plasmodium, ASFV, Salmonella
All prices without logistics costs.