Your alternative to RPA technology

Recombinase Aided Amplification for real-time fluorescence detection

Intended Purpose

(RT-)RAA Nucleic Acid Amplification Kit (Fluorescent Method) can be used to amplify nucleic acids. The amplification result can be verified by use of fluorescent probes in real-time.

 

Advantages

Rapid amplification and real-time detection

RAA technology amplifies RNA and DNA in just a few minutes. Positive amplification results are usually detectable after 3 to 10 minutes incubation, provided an appropriate fluorescence reader is used for incubation.

 

Aliquoted freeze-dried reagent mixes

Recombinase Aided Amplification kits are convient to handle. They comprise of freeze-dried enzyme mix aliquoted in standard 0.2mL PCR tubes and of two buffers for rehydration and starting the amplification reaction.

 

Reverse transcriptase included

In the RT-version of RAA kits, the reverse transcriptase enzyme is included in the freeze-dried reagent pellet. There is no extra pipetting step and no extra costs.

 

Recombinase Aided Amplification Kit (RAA and RPA)

 

Recombinase Aided Amplification (RAA) kits are manufactured by Jiangsu Qitian Gene Biotechnology Co., Ltd.

How to use

Protocol for fluorescent RAA assays:

  • prepare mastermix of reagents buffer, magnesium acetate and primer-probe mix
  • open tube with freeze-dried reagents and add mastermix into the inner surface of the tube lid
  • add extracted nucleic acids to the lid as well
  • close tube, short-spin and mix by pulse-vortexing
  • place tube in your instrument and start incubation and detection (at approx. 37-42°C)

 

Virus-specific real-time detection assays available for:

SARS-CoV-2 Japanese Encephalitis Virus
African Swine Fever (ASF) Virus Ebola Virus
Yellow Fever Virus Influenza A Virus
Zika Virus Dengue Virus

 

Further real-time detection assays available for:

Mycobacterium tuberculosis Vibrio cholerae
Plasmodium spp. Vibrio parahemolyticus
Salmonella spp. Bacillus anthracis
Listeria monocytogenes Yersinia pestis
Staphylococcus aureus Brucella spp.
Enterobacter sakazakii  

 

 

 

 

How to order

Prices and Accessories

F00001

RAA kit for DNA amplification and real-time fluorescence detection

48 reactions

175.00€

F00R01

RT-RAA kit for RNA amplification and real-time fluorescence detection

48 reactions

238.00€

RAA assays

Real-time assays for numerous pathogens, e.g. SARS-CoV-2, MTB, Dengue, Plasmodium, ASFV, Salmonella

48 reactions

685.00€

P003843

Axxin T8 ISO

Portable isothermal fluorescence reader with 2 fluorescence channels

on request

SSB-50

High-quality stainless-steel balls – required to utilize the automated mixing function of the T8-ISO instrument. Sufficient for 50 amplifications. Including tweezers for easier handling.

28.90 €

 

All prices without logistics costs.

Scientific literature

References

Shen et al. (2019) BMC Infect Dis 19: 229

A rapid and sensitive recombinase aided amplification assay to detect hepatitis B virus without DNA extraction

Duan et al. (2018) Biotechniques 64(5): 211

A probe directed recombinase amplification assay for detection of MTHFR A1298C polymorphism associated with congenital heart disease

Chen et al. (2018) Diagn Microbiol Infect Dis 90(2): 90

Use of a rapid reverse-transcription recombinase aided amplification assay for respiratory syncytial virus detection

Zhang et al. (2017) J Microbiol Methods 139: 202

Rapid detection of Salmonella with Recombinase Aided Amplification

Zheng et al. (2015) Academia J Biotechnol 3(3): 27

Rapid detection of Yellow fever Virus with Recombinase Aided Amplification

Li et al. (2022) Biomed Environ Sci 35(2): 133

Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus