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Recombinase Aided Amplification for real-time fluorescence detection
Intended Purpose
(RT-)RAA Nucleic Acid Amplification Kit (Fluorescent Method) can be used to amplify nucleic acids. The amplification result can be verified by use of fluorescent probes in real-time.
Recombinase Aided Amplification (RAA) kits are manufactured by QT Biotech Co., Ltd.
Advantages of Recombinase Aided Amplification (RAA)
Rapid amplification and real-time detection
RAA technology amplifies RNA and DNA in just a few minutes. Positive amplification results are usually detectable after 3 to 10 minutes incubation, provided an appropriate fluorescence reader is used for incubation.
Aliquoted freeze-dried reagent mixes
Recombinase Aided Amplification kits are convient to handle. They comprise of freeze-dried enzyme mix aliquoted in standard 0.2mL PCR tubes and of two buffers for rehydration and starting the amplification reaction.
Reverse transcriptase included
In the RT-version of RAA kits, the reverse transcriptase enzyme is included in the freeze-dried reagent pellet. There is no extra pipetting step and no extra costs.
How to use
Protocol for fluorescent RAA assays:
- prepare mastermix of reagents buffer, magnesium acetate and primer-probe mix
- open tube with freeze-dried reagents and add mastermix into the inner surface of the tube lid
- add extracted nucleic acids to the lid as well
- close tube, short-spin and mix by pulse-vortexing
- place tube in your instrument and start incubation and detection (at approx. 37-42°C)
Virus-specific real-time detection assays available for:
| SARS-CoV-2 | Influenza A Virus |
| Monkey Pox Virus | HIV-1 |
| MERS Coronavirus | Dengue Virus |
| West Nile Virus | Rift Valley Fever Virus |
| Yellow Fever Virus | Zika Virus |
| Chikungunya Virus | Marburg Virus |
| Lassa Fever Virus | Ebola Virus |
| African Swine Fever Virus | Japanese Encephalitis Virus |
Further real-time detection assays available for:
| Mycobacterium tuberculosis | Vibrio cholerae |
| Plasmodium spp. | Vibrio parahemolyticus |
| Schistosoma haematobium | Salmonella spp. |
| Schistosoma mansoni | Listeria monocytogenes |
| Schistosoma japonicum | Brucella spp. |
| Staphylococcus aureus | Enterobacter sakazakii |
| Bacillus anthracis | Yersinia pestis |
How to order
Prices and Accessories
F00001
RAA kit for DNA amplification and real-time fluorescence detection
48 reactions
185.00€
F00R01
RT-RAA kit for RNA amplification and real-time fluorescence detection
48 reactions
248.00€
RAA assays
Real-time assays for numerous pathogens, e.g. SARS-CoV-2, MTB, Dengue, Plasmodium, ASFV, Salmonella
48 reactions
685.00€
P003843
Axxin T8 ISO
Portable isothermal fluorescence reader with 2 fluorescence channels
on request
SSB-50
High-quality stainless-steel balls – required to utilize the automated mixing function of the T8-ISO instrument. Sufficient for 50 amplifications. Including tweezers for easier handling.
28.90 €
All prices without logistics costs.
